Sounds like you ran out of stored fuel and were knackered.
Well, while asking on here, I’ve had a quick google for lactic threshold.
Seems, based on my ride yesterday, that yes, I may have depleted most of the glycogen in the muscles I was using.
But it also seems that as a function of using that stored energy, that there must have been lactic acid present I the muscle ?.
Also, when I reached this point, a feeling I became aware of.
That I couldn’t go as hard, anymore, but for short bursts.
Then the 50/50 may have assisted ?.