Never done it, so have no real-world advice. But, as someone who probably will do it at some point … here’s some stuff to think about …
Gearing – I’d rule out those two climbs from being the determining factor; the key issue is what gearing can you cope with on an uppy/downy route for 12-15 hours over 150 miles. You might be ok with a 29t easiest gear for 60 miles, but an all dayer might be something else?
Speed – Might only be 1km/h difference on your 25km route, but factor in weight and rolling resistance, and increased climbing, over 150 miles, and the difference is likely to be greater. If it’s 2kmh difference, that could easily be an hour or more off your total riding time.
Comfort, which bike is most comfortable / less fatiguing for a long day out? If it rains, does either bike run (or able to run) mudguards?
I’d take the carbon bike.
Or maybe the steel bike …