All seem the same in use and I could not tell one set from the other in use personally.
Can people really tell/care?
Depends on the length of the ride I think.
Due to wash cycles I wear a wide variety of lycra for my commute: dhb boxer undershorts, or cheap Endura liner shorts most of the time, Endura fs260 bibs when its a bit cold and dhb Vaeon Roubaix 3/4s when it is proper cold.
I can tell the difference in comfort, but my commute is only 11 miles each way so it doesn’t really matter a jot.
On a longer leisure ride (say 30 mile+) I’d reach straight for the fs260s as it has a good thick comfortable pad that I can ride in all day without chamois creams and other witchcraft. (The Vaeon pad is pretty good too if its cold).
Also, despite being a dhb fan, I didn’t get on with the Aeron shorts at all. I found the material was too thin/cheap feeling and the seams weren’t flatlocked so they irritated my skin. Good pad in it though.