I always ask people if they are okay – even though most of the time I’d not be able to help, as my mechanical skills are useless, but I ask anyway. Like Sharki said, manners cost nothing. I think everyone should check, regardless of gender. A few weeks ago I was out and punctured. I was passed by 3 female mtb’ers and a bloke. The females completely ignored me, but the chap asked if I had everything I needed.
I was thinking about this at the weekend – I stopped on a descent and held a gate open for a chap, who ride through without so much as a thank you. Further on down the trail, there was another gate – I wasn’t that far behind him, but he just shut the gate and rode off. Ggggrrr. I’ve come across other incidents – like men/women letting the blokes i’m riding with pass, but not me, even though i’m going faster than them/keeping up with the blokes i’m riding with. I see no reason for it, other than i’m female and they don’t want to be passed by a woman.