I don’t think Mapsource can tell you the total ascent/descent.
But Garmin Basecamp can, you can download it for free.
Or SportTracks. http://www.zonefivesoftware.com/sporttracks/
Or if you just want a simple program, Jasta can tell you this (plus loads of other statistics about the track). http://www.it-pannonia.com/index.php?option=com_content&task=view&id=13&Itemid=34
Note that the accuracy of this can variable. Even if you are going along a flat road, the GPS can still record the elevation as going up or down a few metres. And all of these small fluctuations can add up. I think Basecamp and SportTracks use some sort of smoothing to improve the accuracy a bit.