Modern Excel files are just zip files – make a copy, change the extension to .zip and open it. You’ll probably find it’s embedding an image or something. Delete the offending file, rezip it, and change the extension back to xlsx. Open.
Like I said: make a copy! This usually works, but obviously deleting part of the file might leave you with a non-working copy… It should give you a hint though as to where to look in the original.
Edit for clarity: An xlsx file is a zip of a whole load of different files representing the bits of the worksheet. One of these is probably causing the problem.