What’s the actual problem?? Pain in the knee? Pain or fatigue in the muscle? Where in the muscle (its vast as the name suggests!)? Immediate onset or several minutes in? It’s not really clear from your description…..
Bike set up/previous injuries/your muscle balance & biomechanic/climbing & sprint technique will all have an influence on motor pattern (timing) of muscle firing……pretty hard for a physio to comment with all those variables and not actually having you on hand to assess….
If there’s no pain there’s every chance this is normal…..lower VM (vastus medialis obliquis) has a role in countering the natural outwards pull on kneecap and normally fires early to counter this. Long adductors sitting on inside of thigh next to vastus medialis are key power muscles in downward stroke particularly if seated….they will fatigue with hard seated work.
Long and short….find a postgrad sports Physio with a cycling interest and get a proper assessment of yours and the bikes setup!